| Aawaz Institute of Media & Management Sciences (AIMS) | Private |
| Advance Health Sciences Institute of Nursing | Private |
| Aga Khan University | Private |
| Al Ghazali University | Private |
| Al-Hamd College of Professional Education | Private |
| Al-Jadoon College of Nursing & Health Sciences | Private |
| Al-Nimrah College of Nursing & Health Sciences | Private |
| Allied Institute of Nursing & Health Sciences | Private |
| Al-Tibri Medical College | Private |
| Ayaz Samoon College of Nursing (Female) Lyari Karachi | Government |
| Bahria University Medical College | Private |
| Bakhtawar Mannar Institute of Nursing | Private |
| British College of Nursing | Private |
| Baqai University | Private |
| Benazir Bhutto Shaheed University, Lyari | Government |
| CAMS College of Business & Information Technology | Private |
| Canadian College of Science & Technology | Private |
| College of Banking & Finance | Private |
| College of Management & Sciences | Private |
| College of Nursing (Male) Korangi Karachi | Government |
| College of Nursing (Male) Liaquatabad Karachi | Government |
| College of Nursing (Male) Lyari Karachi | Government |
| College of Nursing Rangers Hospital | Private |
| Commecs Institute of Business and Emerging Sciences | Private |
| City Institute of Health Sciences | Private |
| Dr. Ruth K.M Pfao College of Nursing (Female) Karachi | Government |
| Dadabhoy Institute of Higher Education | Private |
| Dawood University of Engineering & Technology | Government |
| DHA Suffa University | Private |
| DHACSS College for Women | Private |
| Dow University of Health Sciences | Government |
| Fountain College of Nursing & Allied Health Sciences | Private |
| Emaan Institute of Management & Sciences | Private |
| Fazaia Ruth Pfao Medical College | Private |
| Federal Urdu University of Arts, Science & Technology | Government |
| Greenwich University | Private |
| Habib University | Private |
| Hamdard University | Private |
| Hands-Institute of Development Studies | Private |
| Hayat Institute of Rehabilitation Medicine | Private |
| Horizon School of Nursing & Health Sciences | Private |
| Ilma University | Private |
| Indus College of Nursing & Midwifery | Private |
| Indus Hospital & Health Network | Private |
| Indus University | Private |
| Indus Valley School of Art & Architecture | Private |
| Institute of Business Administration Karachi (IBA) | Government |
| Institute of Business Management (IoBM) | Private |
| Institute of Space Technology (IST) | Private |
| Iqra University | Private |
| Jinnah Medical & Dental College | Private |
| Jinnah Sindh Medical University | Government |
| Jinnah University For Women (JUW) | Private |
| Karachi Institute of Economics & Technology (KIET) | Private |
| Karachi Institute of Economics & Telecommunication (KITE) | Private |
| Karachi Institute of Health Sciences | Private |
| Karachi Institute of Management & Sciences | Private |
| Karachi Institute of Medical Sciences | Private |
| Karachi Institute of Nursing & Allied Health Sciences | Private |
| Karachi School of Business & Leadership | Private |
| KASB Institute of Technology (KASBIT) | Private |
| Kharadar General Hospital School of Nursing | Private |
| Liaquat College of Medicine & Dentistry | Private |
| Liaquat National Hospital & Medical College | Private |
| Life Saving Institute of Nursing | Private |
| Malir University of Science & Technology | Private |
| Memon Medical Institute Hospital | Private |
| Millennium Institute of Technology & Entrepreneurship (MITE) | Private |
| Muhammad Ali Jinnah University (MAJU) | Private |
| Murshid School of Nursing & Midwifery | Private |
| National Institute of Child Health (NICH) | Government |
| National Institute of Physical Therapy & Rehabilitation Sciences (NIPARS) | Private |
| National Textile University (NTU) | Government |
| FAST University (NUCES) | Private |
| National University of Modern Languages (NUML) | Government |
| Naz Nursing Institute of Health Sciences | Private |
| Nazeer Hussain University | Private |
| Newport Institute of Communications & Economics | |
| Notre Dam Institute of Education | Private |
| NED University of Engineering & Technology | Government |
| Oxford College of Nursing & Allied Health Sciences | Private |
| Pakistan Institute of Rehabilitation & Health Sciences | Private |
| PALPA Institute of Aviation & Management Sciences | Private |
| Patel Institute of Nursing & Allied Health Sciences | Private |
| Peace Institute of Nursing & Health Sciences | Private |
| Preston University | Private |
| Qadri College of Health Sciences | Private |
| Qatar College of Nursing (Female) Karachi | Government |
| Rehman Institute of Nursing & Health Sciences | Private |
| Saifee Burhani School of Nursing | Private |
| Saifee Burhani University | Private |
| Salim Habib University | Private |
| School of Ordinance Malir Cantt | Public |
| Shaheed Benazir Bhutto City University | Private |
| Shaheed Benazir Bhutto Dewan University | Private |
| Shaheed Zulfiqar Ali Bhutto Institute of Science and Technology (SZABIST) | Private |
| Shaheed Zulfiqar Ali Bhutto Law College | Government |
| Shaheed Zulfiqar Ali Bhutto University of Law | Government |
| Sheikh Zayed Islamic Centre, University of Karachi | Government |
| Sindh Institute of Management & Technology | Private |
| Sindh Institute of Medical Sciences (SIMS) – SIUT | Government |
| Sindh Institute of Physical Medicine and Rehabilitation | Government |
| Sindh Madressatul Islam University | Government |
| Sir Adamjee Institute of Management Sciences | Private |
| Sir Syed College of Medical Sciences for Girls | Private |
| Sir Syed University of Engineering & Technology (SSUET) | Private |
| Sohail University | Private |
| St James School of Nursing & Health Sciences | Private |
| Svastu School of Nursing & Health Sciences | Private |
| Tabba Heart Institute | Private |
| Taqwa Institute of Physiotherapy & Health Sciences | Private |
| Textile Institute of Pakistan (TIP) | Private |
| UIT University | Private |
| United College of Physical Therapy | Private |
| United Medical & Dental College | Private |
| University of Karachi | Government |
| Ziauddin University | Private |